Document Type : Original Article
Authors
1
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha,
2
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha
3
Department of Pharmacology, Faculty of Medicine, Benha University, Benha
4
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
5
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt .
Abstract
Introduction: Triclosan [TCS] is a broad-spectrum antibacterial xenoestrogen
commonly used in cosmetics, soap and various products. Exposure to Triclosan
creates a disruption between reactive oxygen species (ROS) and antioxidant defenses.
Oxymatrine [OMT] has potent anti-cancer, anti-fibrosis, and anti-oxidant effects. Aim
of work: To study the effect of Oxymatrine on Triclosan-induced ovarian toxicity.
Material and Methods: The studied rats have been divided into five groups for
measurement of total antioxidant capacity, Caspase-3, the levels of Tumor Necrosis
Factor –alpha[TNF- α], Estrogen and Progesterone levels , and Liposaccharide Binding
Protein [LBP] gene expression in response to Triclosan-induced ovarian toxicity by realtime
Polymerase Chain Reaction [PCR]. Results: Triclolsan [TCS] caused statistically
significant reduction in total antioxidant capacity, with statistically significant elevation
in TNF-α and Caspase-3 activity compared to the control group. Oxymatrine induced
statistically significant elevation in total antioxidant capacity, statistically significant
decrease in the level of TNF-α, Caspase-3 activity with increased levels of Estrogen
and Progesterone compared to the Triclosan group. Histopathological and electron
microscope examination revealed vacuolar degeneration and atretic follicles in rats
treated with Triclosan, and significant improvement after Oxymatrine intake. In the
Triclolsan + Oxymatrine [TCS+OMT] group, gene expression levels of TNF‑α,
Caspase 3, Mitogen-activated protein kinase [MAPK], Nuclear Factor-kappa [NF-KB],
and Liposaccharide Binding Protein [LBP] were statistically significant reduced compared
to the Triclolsan group. Conclusion: The use of Oxymatrine [OMT] as anti-oxidant lead
to decrease in genetic expression of Tumor Necrosis Factor –alpha [TNF‑α] , Caspase 3,
p38-MAPK, [NF-KB], and LBP, in Triclosan-induced ovarian injuries.
Keywords
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