NE, A., HM, A., HA, E., NM, S., AR, M. (2021). EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.. Egyptian Journal of Occupational Medicine, 45(2), 101-116. doi: 10.21608/ejom.2021.170571
Ali NE; Abd El kareem HM; Elnoury HA; Shalaby NM; Mahmoud AR. "EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.". Egyptian Journal of Occupational Medicine, 45, 2, 2021, 101-116. doi: 10.21608/ejom.2021.170571
NE, A., HM, A., HA, E., NM, S., AR, M. (2021). 'EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.', Egyptian Journal of Occupational Medicine, 45(2), pp. 101-116. doi: 10.21608/ejom.2021.170571
NE, A., HM, A., HA, E., NM, S., AR, M. EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.. Egyptian Journal of Occupational Medicine, 2021; 45(2): 101-116. doi: 10.21608/ejom.2021.170571
EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.
1Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha,
2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha
3Department of Pharmacology, Faculty of Medicine, Benha University, Benha
4Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
5Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt .
Abstract
Introduction: Triclosan [TCS] is a broad-spectrum antibacterial xenoestrogen commonly used in cosmetics, soap and various products. Exposure to Triclosan creates a disruption between reactive oxygen species (ROS) and antioxidant defenses. Oxymatrine [OMT] has potent anti-cancer, anti-fibrosis, and anti-oxidant effects. Aim of work: To study the effect of Oxymatrine on Triclosan-induced ovarian toxicity. Material and Methods: The studied rats have been divided into five groups for measurement of total antioxidant capacity, Caspase-3, the levels of Tumor Necrosis Factor –alpha[TNF- α], Estrogen and Progesterone levels , and Liposaccharide Binding Protein [LBP] gene expression in response to Triclosan-induced ovarian toxicity by realtime Polymerase Chain Reaction [PCR]. Results: Triclolsan [TCS] caused statistically significant reduction in total antioxidant capacity, with statistically significant elevation in TNF-α and Caspase-3 activity compared to the control group. Oxymatrine induced statistically significant elevation in total antioxidant capacity, statistically significant decrease in the level of TNF-α, Caspase-3 activity with increased levels of Estrogen and Progesterone compared to the Triclosan group. Histopathological and electron microscope examination revealed vacuolar degeneration and atretic follicles in rats treated with Triclosan, and significant improvement after Oxymatrine intake. In the Triclolsan + Oxymatrine [TCS+OMT] group, gene expression levels of TNF‑α, Caspase 3, Mitogen-activated protein kinase [MAPK], Nuclear Factor-kappa [NF-KB], and Liposaccharide Binding Protein [LBP] were statistically significant reduced compared to the Triclolsan group. Conclusion: The use of Oxymatrine [OMT] as anti-oxidant lead to decrease in genetic expression of Tumor Necrosis Factor –alpha [TNF‑α] , Caspase 3, p38-MAPK, [NF-KB], and LBP, in Triclosan-induced ovarian injuries.
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